Rare Diseases & Orphan Drug

Introduction & Objective: Recent studies had showed that Mesenchymal Stem Cells (MSCs) have beneficial effects on bacterial infections. Treatment with MSCs has proven bacterial clearance. Th is study was undertaken to study the in vitro activity of antimicrobial and antibiofi lm activity of stems cells against gram negative multidrug resistant organism from urinary tract infections.

 

Method: The samples will be processed according to standard protocol following standard guidelines. All the isolates obtained will be identified by standard guidelines. Total of 50 isolates were collected. The antibiotic susceptibility testing will be done for all the isolates by Kirby Bauer disc diffusion method following CLSI guidelines. All the isolates are screened for production of biofi lm by tissue culture plate method. The antimicrobial activity of mesenchymal stem cells was done by micro broth dilution method.

 

Results: Among the 50 gram negative isolates 22 (44%) were Pseudomonas species 12 (24%) were E coli 8 (16%) were Klebsiella spp and 8 (16%) were Proteus species. Among the 50 isolates 32 (64%) were multi drug resistant to the antibiotics tested. Among the 50 isolates 43 (86%) produced biofi lm of which 28 (65%) were strong producer 8 (18%) were moderate biofi lm producer and 7(16.27%) were weak biofi lm producers. All 43 isolates showed sensitivity for the mesenchymal stem cells with MIC range of 32-0.25μg.

 

Conclusion: So far only very few or no studies have been reported on anti-biofi lm activity of mesenchymal stems cells. From our study stem cell, therapy with MSC will be eff ective and alternate for antibiotic resistance in chronic urinary infection there by can serve as therapeutic options for treating drug resistant organisms.